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Application of cryotechniques in cartilage tissue preservation an immunoelectron microscopy: Potentials and problems
Author(s) -
Hunziker Ernst B.
Publication year - 1993
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070240602
Subject(s) - immunoelectron microscopy , extracellular matrix , cartilage , proteoglycan , matrix (chemical analysis) , chemistry , fixation (population genetics) , biophysics , microbiology and biotechnology , materials science , biology , anatomy , biochemistry , immunology , chromatography , immunohistochemistry , gene
Cryotechnical processing of cartilage has the potential to solve many of the tissuespecific problems associated with various routine chemical fixation protocols. This is particularly the case with respect to extracellular matrix architecture, the distortion or destruction of which (caused by extraction and/or precipitation of proteoglycan molecules) may be prevented. Adoption of such techniques also permits high‐sensitivity immunoelectron‐microscopy of the extracellular matrix space (carbohydrate epitopes). However, a number of difficulties still remain to be resolved, particularly that of matrix‐cell interface separation occurring during freeze substitution and low temperature embedding. These problems are briefly addressed and possible solutions outlined. © 1993 Wiley‐Liss, Inc.