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Comparison of nuclear hydration in boar spermatozoa before and after freeze‐thawing: Contrast analysis of cells embedded in bromide‐labeled medium
Author(s) -
Courtens J. L.,
Ekwall H.,
Plöen L.
Publication year - 1992
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070210308
Subject(s) - boar , chromatin , bromide , sperm , chemistry , biophysics , cell nucleus , biology , biochemistry , genetics , organic chemistry , cytoplasm , dna
Epon labeled with bromide was used to embed ejaculated and freeze‐thawed spermatozoa, with the hypothesis that it replaces most of cell water. Image analysis of relative contrasts between sperm nuclei and the surrounding medium revealed that when used in low concentrations, bromide is mostly absorbed to the nuclear structures. For higher concentrations, the chromatin is saturated, and the increase in contrast can be used to calculate relative differences in the hydration of nuclei. Boar sperm nuclei are more hydrated after freeze‐thawing than before. © 1992 Wiley‐Liss, Inc.