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Use of ultrastructural immunohistochemistry in human pituitary pathology
Author(s) -
Sano Toshiaki
Publication year - 1992
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070200204
Subject(s) - immunohistochemistry , ultrastructure , pathology , biology , medicine
Recent advances in ultrastructural immunohistochemistry have provided insight into not only the subcellular localization of single antigens but also the colocalization of two distinct antigens in the same cellular constituent. In the field of pituitary pathology, precise identification of cell types, mechanism of processing, and dynamic intracellular transportation of hormones, as well as produciton of multiple hormones in the same cells of nontumorous and neoplastic adenohypophyses, have been documented by use of these techniques. The present review deals with the use of major methods for ultrastructural immunohistochemistry including pre‐, post‐, and non‐embedding methods, paricularly focusing on their application to human pituitary pathology. Problems of tissue processing and a protocol for double labeling technique using the protein A–gold complex are also described.