Premium
Notes on theory and experimental conditions behind two‐photon excitation microscopy
Author(s) -
Esposito Alessandro,
Federici Federico,
Usai Cesare,
Can Fabio,
Chirico Giuseppe,
Collini Maddalena,
Diaspro Alberto
Publication year - 2003
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.10425
Subject(s) - excitation , two photon excitation microscopy , microscopy , optical sectioning , fluorescence microscope , photon , physics , optics , optical microscope , fluorescence , materials science , quantum mechanics , scanning electron microscope
This report deals with the fundamental quantum physics behind two‐photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission. Microsc. Res. Tech. 63:12–17, 2004. © 2003 Wiley‐Liss, Inc.