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Appraisal of Sims applicability to boron studies in plants
Author(s) -
Dérue Cedric,
Gibouin David,
Verdus MarieClaire,
Lefebvre Fabrice,
Demarty Maurice,
Ripoll Camille,
Thellier Michel
Publication year - 2002
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.10125
Subject(s) - boron , isotopes of boron , isotope , chemistry , ion , secondary ion mass spectrometry , radiochemistry , analytical chemistry (journal) , analyser , environmental chemistry , chromatography , nuclear physics , physics , organic chemistry
In the search for a new methodological approach applicable to the determination of the still poorly known primary role of boron in plant physiology, we have undertaken to appraise the potential of the SIMS method for the analytical imaging of the boron isotopes, 10 B and 11 B, at physiological concentrations in plants. With our own, CAMECA IMS4F SIMS ion analyser, and using O 2 + as primary ions for the detection of B + (plus 12 C + and 40 Ca + ) secondary ions, we have been able to map quantitatively the two boron isotopes in control and boron‐enriched plants, to evaluate boron concentrations at the level of individual cells and to determine boron isotopic ratios. This provides the opportunity to carry out the simultaneous labeling and imaging of boron, using enrichment with the stable isotopes, 10 B and 11 B. The method has also the potential for the simultaneous, quantitative detection of the boron isotopes and of the borate‐binding sites in plant cells. Microsc. Res. Tech. 58:104–110, 2002. © 2002 Wiley‐Liss, Inc.