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Screening of supports for the immobilization of pectinmethylesterase from acerola ( Malpighia glabra L)
Author(s) -
de Assis Sandra Aparecida,
Fernandes Pedro,
Ferreira Bruno Sommer,
Cabral Joaquim MS,
Faria Oliveira Olga Maria Mascarenhas
Publication year - 2004
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.973
Subject(s) - chemistry , agarose , sepharose , chromatography , glutaraldehyde , concanavalin a , gelatin , solanum , biochemistry , botany , enzyme , in vitro , biology
A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin‐water (78.0%). Copyright © 2004 Society of Chemical Industry