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Use of response surface methodology to evaluate the optimum environmental conditions for the bioconversion of xylose into xylitol in eucalyptus hemicellulosic hydrolysate
Author(s) -
Almeida e Silva João B,
Canilha Larissa,
Canettieri Eliana V,
Felipe Maria GA,
Nápoles Solenzal Ana Irene
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.888
Subject(s) - xylitol , xylose , hydrolysate , bioconversion , chemistry , food science , ammonium , activated charcoal , ammonium sulfate , fermentation , biochemistry , adsorption , chromatography , organic chemistry , hydrolysis
Candida guilliermondii fermented a eucalyptus hemicellulosic hydrolysate that had been treated with calcium oxide, phosphoric acid and activated charcoal. The influences of different xylose and ammonium sulfate concentrations on the production of xylitol were studied. A response surface was obtained with value R 2 > 0.97 and p < 0.01. The model estimated a maximum xylitol production of 20.82 g dm −3 in the culture medium supplemented with ammonium sulfate (1.8 g dm −3 ) and xylose (45.5 g dm −3 ). Copyright © 2003 Society of Chemical Industry