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Effect of water‐miscible solvents on the organic solvent‐resistant tyrosinase from Streptomyces sp. REN‐21
Author(s) -
RodakiewiczNowak Janina,
Ito Masaaki
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.857
Subject(s) - chemistry , solvent , acetone , aqueous solution , dimethylformamide , tyrosinase , catechol , methanol , acetonitrile , solvent effects , organic chemistry , ethanol , ethyl acetate , organic solvent , nuclear chemistry , inorganic chemistry , chemical engineering , enzyme , engineering
Abstract The effect of various water‐miscible organic solvents (ethanol, methanol, acetone, acetonitrile, N,N ‐dimethylformamide (DMF) and dimethylsulfoxide (DMSO)) on the kinetics of 4‐ tert ‐butylcatechol (tBC) oxidation in the presence of different samples of organic solvent‐resistant tyrosinase (OSRT) has been studied. In contrast to mushroom tyrosinase the enzyme shows a high relative stability in solutions of organic solvents and increased activity toward the bulky and hydrophobic substrate, tBC, in respect to catechol. Rates of the studied OSRT‐catalyzed reactions are however reduced by the presence of organic solvents and for all studied samples of OSRT decrease exponentially with the content of an organic solvent. The effect has been satisfactorily described by the effect of organic solvents on the thermodynamic activity of tBC. The correlation of the inhibition parameters with the hydrophobicity of a particular solvent (log P ), its intrinsic molar volume, V i , and the Dimroth–Reichardt parameter, E T (30), are shown. The results allow also the prediction of OSRT activity in aqueous solutions of water‐miscible organic solvents. Copyright © 2003 Society of Chemical Industry