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Biotransformation of l ‐menthol by twelve isolates of soil‐borne plant pathogenic fungi ( Rhizoctonia solani ) and classification of fungi
Author(s) -
Miyazawa Mitsuo,
Kawazoe Hideki,
Hyakumachi Mitsuro
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.829
Subject(s) - rhizoctonia solani , rhizoctonia , biotransformation , mycelium , substrate (aquarium) , biology , chemistry , botany , microbiology and biotechnology , horticulture , organic chemistry , enzyme , ecology
The microbial transformation of l ‐menthol ( 1 ) was investigated by using 12 isolates of soil‐borne plant pathogenic fungi, Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4, P‐1; AG‐1‐ID RCP‐1, RCP‐3, and RCP‐7) as a biocatalyst. Rhizoctonia solani F‐1, F‐4 and P‐1 showed 89.7–99.9% yields of converted product from 1 , RCP‐1, RCP‐3, and RCP‐7 26.0–26.9% and the other isolates 0.1–12.0%. In the cases of F‐1, F‐4 and P‐1, substrate 1 was converted to (−)‐(1 S ,3 R ,4 S ,6 S )‐6‐hydroxymenthol ( 2 ), (−)‐(1 S,3R,4S) ‐1‐hydroxymenthol ( 3 ) and (+)‐(1 S ,3 R ,4 R ,6 S )‐6,8‐dihydroxymenthol ( 4 ), which was a new compound. Substrate 1 was converted to 2 and/or 3 by RRG97‐1, 110.4, RCP‐1, RCP‐3 and RCP‐7. The structures of the metabolic products were elucidated on the basis of their spectral data. In addition, metabolic pathways of the biotransformation of 1 by Rhizoctonia solani are discussed. Finally, from the main component analysis and the differences in the yields of converted product from 1 , the 12 isolates of Rhizoctonia solani were divided into three groups based on an analysis of the metabolites. Copyright © 2003 Society of Chemical Industry