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Kinetics of lipase‐catalysed interesterification of triolein and caprylic acid to produce structured lipids
Author(s) -
Camacho Paez B,
Robles Medina A,
Camacho Rubio F,
Esteban Cerdán L,
Molina Grima E
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.810
Subject(s) - caprylic acid , triolein , chemistry , interesterified fat , lipase , oleic acid , chromatography , rhizomucor miehei , glyceride , kinetics , triacylglycerol lipase , reaction rate constant , fatty acid , organic chemistry , enzyme , biochemistry , physics , quantum mechanics
The influence of the molar ratio caprylic acid/triolein, enzyme concentration and water content on the kinetics of the interesterification reaction of triolein (TO) and caprylic acid (CA) were studied. The enzyme used was the 1,3‐specific Rhizomucor miehei lipase. Data modelling was based on a simple scheme in which the acid was only incorporated in positions 1 and 3 of the glyceride backbone. In addition, it was assumed that positions 1 and 3 of the triglycerides were equivalent and that the events at position 1 did not depend on the nature of the fatty acid in position 3 and vice versa. Monoglycerides and diglycerides were not detected during the experiments. This was attributed to the low water content of the immobilised enzyme particles. The value of the equilibrium constant, K , for the exchange of caprylic and oleic acids was 2.7, which indicated that the incorporation of caprylic acid into triglycerides was favoured compared with the incorporation of oleic acid. Simple first order kinetics could describe the interesterification reaction. Using this model and the calculated equilibrium constant, the apparent kinetic constants were calculated. The model fitted all the experimental data except for the CA/TO molar ratios larger than 6. Moreover, the interesterification reaction rate had a maximum value at CA/TO molar ratios of 4–6 mol mol −1 . Copyright © 2003 Society of Chemical Industry