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Bioremediation of toluene‐contaminated air using an external loop airlift bioreactor
Author(s) -
Harding Rochelle C,
Hill Gordon A,
Lin YenHan
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.805
Subject(s) - toluene , bioreactor , pseudomonas putida , airlift , biofilter , sparging , bioremediation , biodegradation , chemistry , aeration , contamination , microbial consortium , chromatography , bioaugmentation , environmental chemistry , air sparging , pulp and paper industry , environmental engineering , environmental science , environmental remediation , microorganism , organic chemistry , biology , bacteria , ecology , genetics , engineering , enzyme
Abstract An external loop airlift bioreactor (ELAB) has been used to capture and degrade toluene from a contaminated air stream. Using a spinning sparger, the toluene could be transferred from small, uniform bubbles into the aqueous culture media with an overall mass transfer coefficient as high as 1.1 h −1 . Due to the very volatile nature of toluene, Pseudomonas putida (ATCC 23973) was cultured and maintained on benzyl alcohol, the first intermediate compound in the metabolic degradation pathway for toluene. Consequently, before successful continuous operation of the ELAB with toluene‐contaminated air, Pseudomonas putida was acclimatized to toluene by using 30 min intermittent sparging of contaminated air into the bioreactor. Continuous sparging of toluene‐contaminated air could then be successfully carried out with 100% capture and biodegradation efficiency at a contaminated air concentration of 15 mg dm −3 and a loading rate of 35 mg dm −3 h −1 . Higher concentrations and loading rates were only partially degraded. Although this capture matches only the low rates reported earlier using biofilters to remediate toluene, the ELAB operates using submerged culture and requires no packing which can plug during biofilter operation. In this study, Pseudomonas putida grew on toluene at a maximum specific growth rate of only 0.05 h −1 . © 2003 Society of Chemical Industry