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Genetic strategy for solving chemical engineering problems in biochemical engineering
Author(s) -
Yu Huimin,
Shi Yue,
Yin Jin,
Shen Zhongyao,
Yang Shengli
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.770
Subject(s) - fermentation , raw material , hydrolysate , starch , metabolic engineering , lysis , chemistry , escherichia coli , biomass (ecology) , lytic cycle , food science , carbon source , biochemistry , industrial microbiology , microbiology and biotechnology , gene , biology , organic chemistry , genetics , agronomy , virus , hydrolysis
Genetic manipulation can be used to solve some traditional chemical engineering problems in biochemical engineering such as in poly(β‐hydroxybutyrate) (PHB) production. To reduce the high production costs of PHB resulting from expensive raw materials, low productivity of the process and complexity of recovery steps, PHB biosynthetic genes ( phb CAB), Vitreoscilla globin gene ( vgb ) and lytic genes of phage λ with an S amber mutation ( S − RRz ) were simultaneously introduced into the same recombinant Escherichia coli , VG1 (pTU14). This will have the advantages of using phb CAB with cheap raw materials and accumulating a high content of PHB, with the ability of vgb to enable a higher specific oxygen uptake rate by the cells, and the characteristics of S − RRz to realize controllable cell lysis. In the fermentation of VG1 (pTU14), starch hydrolysate was used in place of glucose as a cheaper carbon source, and a PHB concentration and productivity as high as 167.6 g dm −3 and 3.05 g dm −3 h −1 were easily realized at almost zero levels of dissolved oxygen (DO), and with a simple temperature‐inducing treatment for recovery PHB with a purity of at least 95% was easily harvested. © 2003 Society of Chemical Industry