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Impact of the presence of solids on peroxidase‐catalyzed treatment of aqueous phenol
Author(s) -
Wagner Monika,
Nicell James A
Publication year - 2003
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.754
Subject(s) - bentonite , phenol , chemistry , peat , aqueous solution , cellulose , peroxidase , nuclear chemistry , hydrogen peroxide , phenols , organic chemistry , chemical engineering , enzyme , biology , ecology , engineering
The impact of the presence of solids on the treatment of aqueous solutions of phenol using horseradish peroxidase (HRP) and hydrogen peroxide was investigated. The solids studied were silica gel, kaolin, bentonite, cellulose and peat moss. Kaolin, bentonite, cellulose and peat moss enhanced phenol transformation at pH 5.0 and 7.0 starting at concentrations of 100, 1000 or 10 000 mg dm −3 . At pH 9.0, bentonite and kaolin had negative impacts when present at 10 000 mg dm −3 and peat moss when present at 1000 mg dm −3 and 10 000 mg dm −3 . In the case of bentonite and peat moss, the enhancing effects at pH 7.0 were associated with the dissolved or colloidal constituents, while in the case of kaolin, the enhancing effects were due to the solid material. Freshly made bentonite suspensions inactivated the peroxidase enzyme; however aged bentonite suspensions and their supernatants did not affect enzyme stability. H 2 O 2 was unstable in solutions containing peat moss constituents. Phenolic solutions treated in the presence of bentonite, kaolin and peat moss were significantly less toxic than the controls, indicating that these materials were able to interact with and partially neutralize precursors of toxic reaction products. Copyright © 2003 Society of Chemical Industry