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Composition control of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) copolymerization by oxygen transfer rate ( OTR ) in Azotobacter vinelandii OPNA
Author(s) -
TorresPedraza Angie Johanna,
SalgadoLugo Holjes,
Segura Daniel,
DíazBarrera Alvaro,
Peña Carlos
Publication year - 2021
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6825
Subject(s) - azotobacter vinelandii , polyhydroxybutyrate , copolymer , chemistry , composition (language) , monomer , oxygen , food science , polyhydroxyalkanoates , nuclear chemistry , bioreactor , polymer chemistry , polymer , organic chemistry , bacteria , biology , nitrogenase , linguistics , philosophy , nitrogen fixation , genetics , nitrogen
BACKGROUND The aim of this study was to evaluate changes in the composition of poly‐3‐hydoxybutyrate‐co‐3‐hydroxyvalerate (PHBV) by the polyhydroxybutyrate (PHB) overproducing mutant OPNA of Azotobacter vinelandii cultured under different conditions. RESULTS The oxygen transfer rate (OTR) significantly affected PHBV composition in cultures in shaken flasks; the highest content of 3‐hydroxyvalerate (3HV; 28%) was achieved from cultures developed at an OTR max of 5.87 mmol L −1 h −1 . In the bioreactor, cultures were grown under oxygen‐limited conditions and different OTRs, varying the agitation rate. The highest PHBV accumulation (85%) was achieved at a low OTR max (4.96 mmol L −1 h −1 ), whereas the maximum content of 3HV (8.5 mol%) was obtained at a higher OTR max (20.3 mmol L −1 h −1 ). The OPNA strain produced a copolymer of ultra‐high molecular weight. CONCLUSIONS Our results reveal that increasing the OTR max raises the molar fraction of 3HV in the PHBV copolymer. Therefore, the manipulation of OTR could be a feasible strategy to produce PHBV copolymers with different monomeric compositions at industrial level. © 2021 Society of Chemical Industry (SCI).