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Stability evaluation of interdigitated liposomes prepared with a combination of 1, 2‐d istearoyl ‐sn‐glycero‐3‐phosphocholine and 1, 2‐dilauroyl ‐sn‐glycero‐3‐phosphocholine
Author(s) -
Adrar Nabil,
Bahadori Fatemeh,
Ceylan Fatma D,
Topçu Gülaçtı,
Bedjou Fatiha,
Capanoglu Esra
Publication year - 2021
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6793
Subject(s) - liposome , differential scanning calorimetry , phosphatidylcholine , phosphocholine , chemistry , dispersity , fourier transform infrared spectroscopy , thermal stability , chromatography , phospholipid , nuclear chemistry , stereochemistry , analytical chemistry (journal) , chemical engineering , organic chemistry , membrane , biochemistry , thermodynamics , physics , engineering
BACKGROUND In order to obtain small and homogenous liposomes encapsulating gallic acid (GA) with interdigitated leaflets, a combination of phosphatidylcholine (PC) of medium length sn‐1 and sn‐2 acyl chains with PC of longer acyl chains at both sn1 and sn2 positions were employed. Four formulations respectively consisting of 1,2‐distearoyl‐sn‐glycero‐3‐phosphocholine (DSPC); DSPC/1,2‐dilauroyl‐sn‐glycero‐3‐phosphocholine (DSPC/DLPC) (1:1, mol mol −1 ); DSPC/DLPC (3:1, mol mol −1 ) and Lipoid S75, were prepared. For each formulation, the stability of liposomes during storage was investigated. RESULTS The initial size of liposomes ranged from 36.61 ± 0.61 to 125.43 ± 2.15 nm and their polydispersity index (PDI) from 0.14 ± 0.01 to 0.28 ± 0.01. The formulations combining DSPC and DLPC resulted in very stable liposomes physically, during up to 84 days of storage with no significant change in both size and PDI except a slight increase in the size at the 42nd day. The molecular state of the bilayers and their thermal behavior as given by FTIR and Differential Scanning Calorimetry (DSC) results supported the hypothesis of an interdigitated state of the liposomes formed by DSPC/DLPC combination at a molar ratio of 3:1 . CONCLUSION Interdigitated liposomes composed of two symmetric phospholipids (DSPC and DLPC) were obtained for the first time and exhibited a good stability during storage in solution. © 2021 Society of Chemical Industry (SCI).