Premium
Continuous culture of Nitrosomonas eutropha and biomethanol production using whole cell biotransformation
Author(s) -
Sabale Tushar,
Babu Mohan,
Panchwagh Shruti,
Ghosalkar Anand
Publication year - 2021
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6661
Subject(s) - biotransformation , nitrosomonas europaea , nitrite , biomass (ecology) , chemistry , ammonia , bioreactor , nitrification , trimethylamine , ammonium , raw material , continuous production , food science , biochemistry , organic chemistry , nitrate , biology , enzyme , environmental engineering , nitrogen , environmental science , agronomy
BACKGROUND Nitrosomonas eutropha is a chemolithoautotrophic organism with an ability to oxidize ammonium to nitrite and nitrate. Continuous culture with and without cell recycling was used to enhance production of N. eutropha cell biomass using a 40‐L bioreactor. Whole cell biotransformation was used to convert methane (CH 4 ) present in the biogas into biomethanol using N. eutropha.RESULTS The maximum biomass production rate of N. eutropha was increased by three‐fold with provision of cell recycling in the continuous culture. Removal of nitrite from the continuous culture resulted in high activity of ammonia monooxygenase enzyme responsible for conversion of CH 4 to biomethanol. Use of ammonium sulfate as a source of electrons for biotransformation reaction with N. eutropha resulted in a maximum 119.86 mg L −1 of biomethanol production. CONCLUSION Our research provides an efficient process for biomass production of N. eutropha using continuous culture. We have demonstrated improvement in CH 4 to biomethanol conversion using biogas as feedstock. © 2021 Society of Chemical Industry