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Recombinant l‐ asparaginase production using Pichia pastoris ( MUT s strain): establishment of conditions for growth and induction phases
Author(s) -
PillacaPullo Omar,
Rodrigues David,
SánchezMoguel Ignacio,
Lopes André,
Pimenta Marcela,
Basi Tajindar,
Feitosa Valker,
Zavaleta Amparo,
Monteiro Gisele,
Pessoa Jr Adalberto,
Vitolo Michele
Publication year - 2021
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6540
Subject(s) - pichia pastoris , bioprocess , bioreactor , glycerol , chemistry , laboratory flask , escherichia coli , recombinant dna , strain (injury) , food science , growth medium , yeast extract , saccharomyces cerevisiae , yeast , methanol , chromatography , biochemistry , biology , bacteria , fermentation , organic chemistry , anatomy , gene , paleontology , genetics
Abstract BACKGROUND l‐ asparaginase (ASNase), a biopharmaceutical enzyme used in the treatment of childhood lymphoid malignancies, is commercially produced from Escherichia coli and Erwinia chrysanthemi. However, it causes severe adverse effects due to allergenic prokaryotic epitopes on the protein surface. ASNase II from Saccharomyces cerevisiae can be a promising alternative source of this enzyme. In this study, conditions to produce ASNase from S. cerevisiae expressed in Pichia pastoris have been investigated in shake flasks and 3 L‐ bioreactor. We evaluated if medium composition, concentration of carbon source (i.e. glycerol), growth time, concentration of inducer (i.e. methanol), temperature and initial pH influenced both biomass and ASNase expression. RESULTS Biomass of around 53 g L –1 and ASNase volumetric activity of 710 U L –1 were achieved using the buffered glycerol ‐ complex medium (BMGY) containing 40 g L –1 glycerol, with induction after 141 h using 3.0% (v/v) methanol, at 20 °C and initial pH 6.0. CONCLUSION The experiments performed in shake flasks were scalable to a 3 L‐ bioreactor, suggesting that this bioprocess could be scaled‐up for industrial production. © 2020 Society of Chemical Industry (SCI)