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Purification and characterization of a novel antioxidant Phelligridin LA produced by Inonotus baumii
Author(s) -
Wang Tianwen,
Li Hui,
Liang Chen,
Sun Shiwei,
Liu Ao,
Zhu Hu
Publication year - 2020
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6430
Subject(s) - dpph , abts , chemistry , antioxidant , context (archaeology) , metabolite , fermentation , inonotus obliquus , ingredient , scavenger , food science , free radical scavenger , antifungal , secondary metabolite , biochemistry , biology , microbiology and biotechnology , paleontology , gene
BACKGROUND The fruit body of medicinal fungus, Sanghuang ( Inonotus baumii ), is a source of diverse metabolites with health‐promoting efficacy through varying mechanisms. The low efficiency of fruit body production and failure to discover valuable metabolites have severely limited more extensive applications. RESULTS A novel compound was discovered and identified as (6,6′‐bis(( E )‐3,4‐dihydroxystyryl)‐4,4′‐dihydroxy‐2 H ,2′ H ‐[3,3′‐bipyran]‐2,2′‐dione (named as Phelligridin LA, PLA). The compound exhibited antioxidant activity against free radicals (DPPH•, •OH, •O 2 , and ABTS• + ). The IC 50 values of PLA and vitamin C were determined as 18.62 and 11.26 μmol L −1 (DPPH•), 222.56 and 718.48 μmol L −1 (•OH), 611.41 and 340.11 μmol L −1 (•O 2 ), 73.33 and 44.31 μmol L −1 (ABTS• + ), which demonstrated that PLA was an efficient scavenger of •OH radical. CONCLUSION PLA is a novel antioxidant metabolite purified from the fermentation broth of Sanghuang, which proved that the biomass produced with fermentation was a good source for metabolite isolation. A feasible process was established to obtain this valuable ingredient from the medicinal fungus. This report exemplifies the exploitation of Sanghuang in the context of modern natural product discovery. © 2020 Society of Chemical Industry