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Two‐step fractionation of Pinus radiata by autohydrolysis and organosolv delignification for enzymatic hydrolysis
Author(s) -
Santos Tamara M,
Rigual Victoria,
Oliet Mercedes,
Alonso M Virginia,
Domínguez Juan C,
Rodriguez Francisco
Publication year - 2019
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.6197
Subject(s) - organosolv , enzymatic hydrolysis , chemistry , pinus radiata , hydrolysis , lignocellulosic biomass , cellulose , softwood , fractionation , lignin , sulfuric acid , chromatography , raw material , pulp and paper industry , organic chemistry , botany , biology , engineering
BACKGROUND Lignocellulosic biomass is a promising renewable feedstock to obtain fuels and chemicals. However, a suitable pretreatment is required to separate its main components and overcome its structural resistance to enzymatic hydrolysis. In this work, a sequential pretreatment composed of autohydrolysis (AH) and organosolv delignification (ORG) is used to fractionate Pinus radiata wood and improve the enzymatic hydrolysis. RESULTS The effect of three different AH pretreatment severities on the delignification degree of organosolv solid fractions was evaluated. In addition, enzymatic hydrolysis was performed to determine the efficiency of the combination of pretreatments. The results showed that a pretreated solid with high cellulose content (88%, w/w) and high delignification degree (88%) was achieved under mild conditions of AH (150 °C and 30 min) and acid‐catalyzed ORG (185 °C, 75 min, 50% (w/w) ethanol and 1% (w/w) sulfuric acid), obtaining an enzymatic hydrolysis yield of 63%. CONCLUSION This study provides the most favorable conditions to fractionate a softwood, P. radiata , employing a two‐step pretreatment based on AH followed by ORG. © 2019 Society of Chemical Industry