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Production of L ‐methionine by immobilized pellets of Aspergillus oryzae in a packed bed reactor
Author(s) -
Yuan YingJin,
Wang Shuhao,
Song Zhengxiao,
Gao Ruichang
Publication year - 2002
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.615
Subject(s) - aspergillus oryzae , pellets , chemistry , packed bed , substrate (aquarium) , chromatography , nuclear chemistry , methionine , enzyme , organic chemistry , materials science , biochemistry , biology , amino acid , composite material , ecology
Production of L ‐methionine by immobilized pellets of Aspergillus oryzae in a packed bed reactor was investigated. Based on the determination of relative enzymatic activity in the immobilized pellets, the optimum pH and temperature for the resolution reaction were 8.0 and 60 °C, respectively. The effects of substrate concentration on the resolution reaction were also investigated and the kinetic constants (K m and V m ) of immobilized pellets were found to be 7.99 mmol dm −3 and 1.38 mmol dm −3 h −1 , respectively. The maximum substrate concentration for the resolution reaction without inhibition was 0.2 mol dm −3 . The L ‐methionine conversion rate reached 94% and 78% when substrate concentrations were 0.2 and 0.4 mol dm −3 , respectively, at a flow rate of 7.5 cm 3 h −1 using the small‐scale packed bed reactor developed. The half‐life of the L ‐aminoacylase in immobilized pellets was 70 days in continuous operation. All the results obtained in this paper exhibit a practical potential of using immobilized pellets of Aspergillus oryzae in the production of L ‐methionine. © 2002 Society of Chemical Industry