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Bioprocess development for (2R,3R)‐butanediol and acetoin production using very high polarity cane sugar and sugarcane molasses by a Bacillus amyloliquefaciens strain
Author(s) -
Maina Sofia,
Mallouchos Athanasios,
Nychas GeorgeJohn E,
Freire Denise MG,
de Castro Aline Machado,
Papanikolaou Seraphim,
Kookos Ioannis K,
Koutinas Apostolis
Publication year - 2019
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5997
Subject(s) - acetoin , cane , fermentation , bioprocess , yield (engineering) , chemistry , 2,3 butanediol , sugar , food science , bacillus amyloliquefaciens , bioreactor , trehalose , substrate (aquarium) , biochemistry , biology , materials science , organic chemistry , paleontology , ecology , metallurgy
BACKGROUND Efficient production of (2R,3R)‐2,3‐butanediol (BDO) and acetoin using the GRAS strain Bacillus amyloliquefaciens from very high polarity (VHP) cane sugar and sugarcane molasses could lead to the development of a sustainable industrial process. RESULTS The effect of initial substrate concentration, nitrogen source, temperature, inoculum size and pH were evaluated in shake flask cultures. Batch bioreactor cultures showed that (2R,3R)‐BDO production (up to 28 g L −1 ) with high yield (up to 0.43 g g −1 ) and productivity (up to 0.93 g L −1 h −1 ) was favored at low k L a values (17–49 h −1 ). Increasing k L a values (63–104 h −1 ) initiated acetoin production (up to 25.6 g L −1 ) with yields up to 0.4 g g −1 and productivities up to 1.42 g L −1 h −1 . Fed‐batch fermentation carried out at k L a value of 49 h −1 with VHP cane sugar resulted in mixed production (127.3 g L −1 ) of (2R,3R)‐BDO, (2R,3S)‐BDO and acetoin at 122 h with high yield (0.50 g g −1 ) and productivity (1.04 g L −1 h −1 ). In a similar fed‐batch fermentation with sugarcane molasses, mainly (2R,3R)‐BDO production (48.7 g L −1 with purity of 87.8%) was achieved at 59 h with yield of 0.4 g g −1 and productivity of 0.83 g L −1 h −1 . At 130 h, (2R,3R)‐BDO was converted mainly into acetoin (55.4 g L −1 ). CONCLUSION This study demonstrates that k L a manipulation could be used in batch cultures to divert the bacterial metabolism towards either (2R,3R)‐BDO or acetoin production. Fed‐batch cultures with sugarcane molasses could lead to either (2R,3R)‐BDO or acetoin production with negligible by‐product formation. © 2019 Society of Chemical Industry