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Bioinspired synthesis of nanofibers on monolithic scaffolds for enzyme immobilization with enhanced loading capacity and activity recovery
Author(s) -
Li Weiran,
Shi Jiafu,
Zhao Yang,
Tian Yu,
Huo Qian,
Wu Yizhou,
Zhang Shaohua,
Jiang Zhongyi
Publication year - 2019
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5963
Subject(s) - cordierite , chemical engineering , nanofiber , horseradish peroxidase , immobilized enzyme , chemistry , materials science , enzyme , nanotechnology , catalysis , biochemistry , engineering
Abstract BACKGROUND The enzymatic process in continuous flow reactors has become attractive owing to the merits of in‐line purification, improved mixing and mass transfer, etc. However, the construction of continuous flow reactors requires stable and surface‐active monolithic scaffolds for enzyme immobilization. RESULTS In this study, nanofibers of linear polyethyleneimine (LPEI) are formed on the surface of poly(sodium 4‐styrenesulfonate) (PSS)‐treated cordierite honeycomb (named LPEI/PSS cordierite‐H) as monolithic scaffold for enzyme immobilization. The controlled self‐assembly of LPEI ensures the formation of nanofibers (diameters of 20–80 nm) on the surface of cordierite‐H, which roughens the surface and offers many active sites for enzyme loading. The electrostatic interaction between negatively charged enzyme and positively charged LPEI helps the enzyme firmly attach on the surface. The loading capacity of LPEI/PSS cordierite‐H was 1.24 mg g −1 . Horseradish peroxidase (HRP)‐loaded LPEI/PSS cordierite‐H (HRP@LPEI/PSS cordierite‐H) exhibited activity recovery of 55.33%, 2‐fold higher than that of HRP‐loaded branched polyethyleneimine (BPEI)/PSS cordierite‐H (HRP@BPEI/PSS cordierite‐H) (23.72%). Furthermore, a silica layer was formed on the surface of HRP@LPEI/PSS cordierite‐H through LPEI‐induced silicification, which could prevent the enzyme from deactivation under extreme conditions. CONCLUSION These monolithic biocatalysts have great potential for applications in wastewater treatment and even in other fields such as pharmaceutical synthesis. © 2019 Society of Chemical Industry