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Bacterial hyaluronic acid production through an alternative extraction method and its characterization
Author(s) -
Güngör Gökhan,
Gedikli Serap,
Toptaş Yağmur,
Akgün Dilber E,
Demirbilek Murat,
Yazıhan Nuray,
Aytar Çelik Pınar,
Denkbaş Emir B,
Çabuk Ahmet
Publication year - 2019
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5957
Subject(s) - fourier transform infrared spectroscopy , gel permeation chromatography , extraction (chemistry) , chemistry , yield (engineering) , hyaluronic acid , attenuated total reflection , biomaterial , chromatography , molar mass distribution , nuclear chemistry , permeation , incubation , proton nmr , infrared spectroscopy , materials science , biochemistry , organic chemistry , membrane , chemical engineering , polymer , biology , engineering , genetics , metallurgy
BACKGROUND In this study, hyaluronic acid (HA) was produced using Streptococcus equi ssp. equi via an optimized Central Composite Design process, with purification by an alternative extraction method. Determinations of molecular weight, cytotoxicity, viscosity; proton nuclear magnetic resonance (H‐NMR) and Fourier transform infrared‐attenuated total reflection (FTIR‐ATR) analyses were provided. RESULTS The HA yield obtained was up to c . 12 g L −1 under the reaction conditions of pH 7.8, 33 °C incubation temperature, 24 h incubation time and 187 rpm agitation rate. According to gel permeation chromatography results, HA has an average molecular weight of 79 416 Da. FTIR‐ATR analysis showed that the spectrum of the standard HA sample overlapped with the spectrum obtained from this study. CONCLUSION In this study, higher titer production of HA was obtained with a simple and economical sequential process. Additionally, the results indicate that the HA produced is an appropriate biomaterial for medical applications. © 2019 Society of Chemical Industry