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Microwave hydrogravity pretreatment of Sargassum muticum before solvent extraction of antioxidant and antiobesity compounds
Author(s) -
FlórezFernández Noelia,
Casas María P,
Jesús GonzálezMuñoz María,
Domínguez Herminia
Publication year - 2019
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5771
Subject(s) - chemistry , aqueous solution , extraction (chemistry) , trolox , fucoxanthin , chromatography , solvent , fraction (chemistry) , antioxidant , ethanol , food science , carotenoid , organic chemistry , antioxidant capacity
BACKGROUND Microwave hydrodiffusion and gravity (MHG) of Sargassum muticum was proposed both for obtaining an aqueous extract and as pretreatment before ethanol extraction of bioactives. RESULTS No significant differences in composition and properties between the aqueous phase drained during MHG processing at 600 W/7 min (MHG‐1) and at 600 W/5 min + 300 W/5 min (MHG‐2) were observed. The aqueous extract, accounting for less than 0.2% of the initial material, was refined by adsorption onto food grade resins to recover and concentrate the phenolic fraction, further eluted in 68% ethanol. The final product contained more than 50% phlorotannins and the antiradical capacity was higher than that of Trolox. Ethanolic extraction of the dried solids yielded 7% non‐volatile solubles, containing 5% phenolics and 1.2% fucoxanthin. After 12 months at room temperature in the dark, phenolic and fucoxanthin content, and antioxidant properties of the extracts were markedly reduced. The aqueous extracts from MHG‐2 and the ethanolic extracts from the dried solids showed inhibition of lipogenesis in 3TL1 cells when used at 100 µg mL −1 . CONCLUSION MHG processing can be proposed both for the extraction of an aqueous fraction and for the conditioning of seaweed biomass before conventional solvent extraction of bioactives. © 2018 Society of Chemical Industry