Premium
An efficient production of a novel carbohydrate polymer Sphingan WL
Author(s) -
Li Hui,
Li Jing,
Zhou Wanlong,
Jiao Xue,
Sun Yajie,
Shen Yaling,
Qian Jin,
Wang Jiqian,
Zhu Hu
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5705
Subject(s) - laboratory flask , incubation , yeast extract , food science , response surface methodology , chemistry , yeast , plackett–burman design , incubation period , volume (thermodynamics) , chromatography , biochemistry , microbiology and biotechnology , biology , fermentation , organic chemistry , physics , quantum mechanics
BACKGROUND The sphingan WL produced by Sphingomonas sp. WG has great application potential in the food, ink and other industries. To discover optimal culture conditions for WL production, a gradual optimization method including one‐factor design, Plackett‐Burman design and response surface methodology was employed. Furthermore, the possible reason for the enhancement in WL production was analysed by detecting the gene expression levels in WL biosynthesis using quantitative real‐time PCR. RESULTS The results showed that the optimal conditions are as follows: seed age, 15–18 h; incubation time, 72 h; inoculum size, 5%; loading volume, 50 mL of medium in 250‐mL shaking flasks; shaking speed, 175 rpm; initial pH of medium, 7.0; and incubation temperature, 32.5°C. WL production was mainly dependent on the concentration of glucose and yeast extract. The statistically optimized culture medium consisted of 72.40 g L −1 glucose, 3.58 g L −1 yeast extract, 3.00 g L −1 K 2 HPO 4 , 0.10 g L −1 MgSO 4 , and 0.10 g L −1 ZnSO 4 . Interestingly, a high WL production of 39.95 g L −1 , 2.37‐fold the initial production (16.82 g L −1 ), was obtained, which was due to the higher expression level of WL‐related enzymes under these optimized conditions. CONCLUSIONS This study provides useful fundamental information for the development of an efficient process for large‐scale WL production. © 2018 Society of Chemical Industry