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Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second‐generation bioethanol production
Author(s) -
Singh Dilip,
Mehta Preeti,
Saxena Rohit,
Barrow Colin J,
Puri Munish,
Tuli Deepak K,
Mathur Anshu S
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5660
Subject(s) - bioconversion , biofuel , biodiesel , pulp and paper industry , biomass (ecology) , oleic acid , biodiesel production , bioreactor , industrial fermentation , food science , chemistry , fermentation , environmental science , waste management , microbiology and biotechnology , biology , agronomy , biochemistry , organic chemistry , engineering , catalysis
BACKGROUND Efficient conversion of inhibitor containing pentose rich stream into bioethanol or biogas is still challenging due to several technological challenges; however, this stream can be efficiently assimilated into lipids by oleaginous yeasts such as Rhodotorula . In this work, a continuous and nonsterile process was developed for bioconversion of waste streams into lipids for biodiesel using growth‐inhibitor‐tolerant oleaginous yeast. RESULTS An inhibitor‐tolerating property of the strain was used to develop a cyclic and continuous near axenic cultivation process in a 2 L nonsterile bioreactor to enhance valorization of waste streams and to reduce energy requirements for media and vessel sterilization. After media optimization, continuous cultivation resulted in significant increase in biomass (14.90 g L −1 day −1 ) and lipid productivity (6.15 g L −1 day −1 ) as compared to flask (biomass ∼3 g L −1 day −1 and lipid productivity ∼0.6 g L −1 day −1 ) results. Palmitic acid (C16:0) and oleic acid (C18:1) were the major fatty acids, accounting for almost 80% of total lipids, thus making lipid produced in the process suitable for biodiesel production. CONCLUSION Inhibitors present in waste streams successfully maintained near axenic conditions during the cultivation. Maximum dilution rate of 1 was achieved after media optimization, which resulted in complete valorization of nutrients into biomass and lipids with high productivity. © 2018 Society of Chemical Industry