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Practical experiences from the bench‐scale implementation of a bioprocess for fucoxanthin production
Author(s) -
GómezLoredo Alma,
GonzálezValdez José,
GonzálezGonzález Mirna,
Benavides Jorge,
RitoPalomares Marco
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5494
Subject(s) - bioprocess , fucoxanthin , ultrafiltration (renal) , chemistry , process (computing) , biochemical engineering , process engineering , scale (ratio) , microbiology and biotechnology , chromatography , computer science , food science , engineering , chemical engineering , biology , operating system , physics , quantum mechanics , carotenoid
BACKGROUND A laboratory‐scale protocol for the production, primary recovery and partial purification of fucoxanthin via biomass production at low light intensities was previously reported. This proposed approach exploited the use of ethanol salt aqueous two‐phase systems (ATPS) and ultrafiltration (UF) to deliver a 45% total recovery yield if all steps were implemented in a bioprocess. In this study, practical experiences derived from the bench‐scale implementation of the previously characterized lab protocol are presented. RESULTS After implementation of the process, a 47.5% fucoxanthin recovery was obtained, while 87.2% of the contaminant proteins were removed. CONCLUSION The findings reported here provide the required guidelines to facilitate process scale‐up for the potential commercial production and recovery of fucoxanthin. © 2017 Society of Chemical Industry