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Pilot scale production of extracellular thermo‐alkali stable laccase from Pseudomonas sp. S2 using agro waste and its application in organophosphorous pesticides degradation
Author(s) -
Chauhan Prakram Singh,
Jha Bhavanath
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5454
Subject(s) - laccase , chemistry , catechol , enzyme , pesticide , nuclear chemistry , extracellular , alkali metal , enzyme assay , bioreactor , food science , chromatography , organic chemistry , biochemistry , biology , agronomy
Background Laccases are multicopper oxidases that are able to oxidize various aromatic or nonaromatic compounds owing to their multifarious applications. However, till now only a few bacterial laccases have been isolated and characterized. Hence there is an urgent need to study an extracellular thermo‐alkali stable laccase. Results In the present study, an extracellular thermo‐alkali stable laccase was produced from Pseudomonas sp. S2 in a 100 L bioreactor using agro waste (potato peel). Production was 17‐fold higher than in the control. The enzyme (S2LAC) was purified 12.16 ± 1.6‐fold to homogeneity with specific activity of 1089.70 ± 16.8 U mg −1 and molecular mass of 38 kDa. The temperature and pH for maximum enzyme activity were 80 °C and 9.0, respectively. The metal ions Na + , K + , Pb +2 , Ca +2 , Cu +2 and Co +2 enhanced enzyme activity. The purified enzyme showed maximum specificity to Pyrogallol > PPD > L‐DOPA > Hydroquinone. The S2LAC was able to degrade organ‐phosphorous pesticide including dichlorophos, chlorpyrifos, monocrotophos and profenovos upto 45.99 ± 0.3%, 80.56 ± 0.6%, 75.45 ± 1.3%, 81.84 ± 0.6%, respectively, in the absence of any mediator. Conclusion S2LAC produced using agro waste was stable and capable of degrading organophosphorous pesticides making it attractive for industrial applications. © 2017 Society of Chemical Industry