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Protein adsorption in anion exchange resins – effects of polymer grafting, support structure porosity, and protein size
Author(s) -
Zhu Mimi,
Fuks Preston,
Carta Giorgio
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5410
Subject(s) - porosity , grafting , adsorption , ion exchange , polymer , chemical engineering , protein adsorption , ion exchange resin , ion , polymer chemistry , chemistry , materials science , composite material , organic chemistry , engineering
BACKGROUND Anion exchange resins are used extensively for the purification of acidic proteins. Grafting charged polymers to a rigid porous structure has been shown to improve performance under varying conditions. Understanding the underlying mechanisms is important for the optimum design and selection of material properties and operating conditions. RESULTS Positively charged grafted polymers incorporated into a rigid, porous polymeric backbone structure are found to significantly enhance adsorption capacity and kinetics of the proteins bovine serum albumin (BSA, M r ∼ 65 kDa) and thyroglobulin (Tg, M r ∼ 660 kDa) but under different conditions. For the smaller BSA, binding increases with grafted polymer length and content and decreases with salt concentration. For the much larger Tg, binding increases with the addition of some salt for the polymer‐grafted resins but can be lower than that observed for ungrafted resins without added salt. This behavior is caused by diffusional hindrance due to the bound protein. Increasing the length of the grafted polymer or the pore size of the backbone improves the Tg adsorption kinetics. CONCLUSION Protein adsorption is controlled by different mechanisms dependent on polymer grafting, backbone structure, and size of the adsorbed protein. Optimum selection of resin properties and conditions is needed to maximize adsorption capacity and mass transfer kinetics. © 2017 Society of Chemical Industry