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Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex
Author(s) -
Wang Lifang,
Shen Yanbing,
Liu Mengjiao,
Tang Rui,
Wang Min
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5372
Subject(s) - biotransformation , chemistry , solubility , ionic liquid , steroid , side chain , membrane permeability , membrane , alkyl , arthrobacter , cell growth , organic chemistry , biochemistry , enzyme , polymer , catalysis , hormone
BACKGROUND Ionic liquids (ILs) have become increasingly attractive as alternatives for organic solvents. In this study, the influences of ILs on the cellular growth rate, cellular activity, cortisone acetate (CA) conversion, CA solubility, and cell membrane permeability of Arthrobacter simplex CPCC 140451 ( ASP ) were examined. RESULTS Results showed that ILs with ≥3 carbon atoms in their side chains increased CA conversion, CA solubility, and cell membrane permeability of ASP and universally inhibited the cellular growth rate and activity, with ILs with [PF 6 ]‐anion exhibiting the most severe inhibition activity. Inhibition was also more serious using ILs with longer alkyl side chains and at higher concentrations. CA conversion, CA solubility, and leakage of cell inclusions showed higher increase under ILs with [PF 6 ]‐anion than those of ILs with [BF 4 ]‐anion and lower increase for ILs with longer side chains. CONCLUSION This study improved the understanding of the influence and primary selection of imidazolium‐based ILs when applied in steroid biotransformation with Arthrobacter simplex whole cells. The data provided a foundation for the selection of suitable ILs for various applications. © 2017 Society of Chemical Industry