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Production of androstenedione using mutants of Mycobacterium sp
Author(s) -
Egorova Olga V,
Gulevskaya Seraphima A,
Puntus Irina F,
Filonov Andrey E,
Donova Marina V
Publication year - 2002
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.536
Subject(s) - androstenedione , methane sulfonate , mutant , yield (engineering) , chemistry , strain (injury) , sterol , steroid , biochemistry , dehydrogenase , molar ratio , enzyme , gene , biology , cholesterol , androgen , materials science , anatomy , hormone , metallurgy , catalysis
Mycobacterium sp VKM Ac‐1815D strain was able to cleave sterol side chain giving androstenedione (AD) as a major product with a molar yield of 63–68%. Clones having altered resistance to antibacterial agents were selected. After treatment with ethyl methane sulfonate or mitomycin C, mutants were obtained that retained the ability to produce AD from sitosterol with molar yields of 70–75%. A mutant strain able to effectively reduce 3,17‐diketosteroids at C‐17 was selected. The 17β‐hydroxy steroid dehydrogenase activity of its crude extract was twice as high as that found for the parent organism. The approach offers the possibility of obtaining improved and labelled biocatalysts for AD or testosterone production from sterols. © 2002 Society of Chemical Industry