Premium
Improving catalytic activity of laccase immobilized on the branched polymer chains of magnetic nanoparticles under alternating magnetic field
Author(s) -
Xia TingTing,
Lin Wan,
Liu ChunZhao,
Guo Chen
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5325
Subject(s) - laccase , catechol , catalysis , chemistry , immobilized enzyme , polyethylenimine , polymer , reaction rate , magnetic nanoparticles , nanoparticle , nuclear chemistry , amine gas treating , polymer chemistry , chemical engineering , organic chemistry , materials science , nanotechnology , enzyme , transfection , biochemistry , gene , engineering
BACKGROUND Immobilization of laccase may cause more or less mass transfer limitation in practical applications. In order to enhance the reaction rate, there is great interest in developing an effective way to increase the rate of diffusion in reactions catalyzed by immobilized laccase. RESULTS The laccase from Trametes versicolor (p‐diphenol: dioxygen oxidoreductases, EC 1.10.3.2) immobilized on different molecular weight polyethylenimine (PEI) modified amine‐functionalized Fe 3 O 4 nanoparticles [Fe 3 O 4 –NH 2 –PEI (1200/10 000/60 000)–laccase] was separately fabricated. The oxidation reaction rate of catechol catalyzed by Fe 3 O 4 –NH 2 –PEI (1200)–laccase under an alternating magnetic field (600 Hz, 10 Gs) was separately 2.10 times and 1.16 times higher than the control without any external force and with mechanical stirring at 150 rpm. This was a larger increase than for Fe 3 O 4 –NH 2 –PEI (10 000/60 000)–laccase. In addition, the reaction rate catalyzed by Fe 3 O 4 –NH 2 –PEI (1200)–laccase was enhanced as the magnetic field frequency, strength and Fe 3 O 4 –NH 2 –PEI (1200)–laccase or catechol concentration was increased. The immobilized laccase retained 85% of its initial activity after six consecutive operations. CONCLUSION Using an alternating magnetic field was a powerful way to intensify the reaction rate catalyzed by laccase immobilized on branched polymer chains of magnetic nanoparticles and showed potential for large‐scale catalytic reaction. © 2017 Society of Chemical Industry