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Biosynthesis of 4‐isopropylbenzoic acid from α‐pinene by using a mutant strain originated from the α‐pinene degrader Pseudomonas veronii ZW
Author(s) -
Cheng Zhuowei,
Tao Jinguo,
Ye Jiexu,
Li Chao,
Zhang Xiaomin,
Chen Jianmeng
Publication year - 2018
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5319
Subject(s) - mutant , pseudomonas , biochemistry , chemistry , pinene , amino acid , strain (injury) , mutagenesis , bacteria , gene , biology , organic chemistry , genetics , anatomy
BACKGROUND Anthropogenic activities, in particular industrial processes, increase the emissions of volatile organic compounds to the atmosphere. Using special microorganisms to achieve resource recovery is a challenge for pollutant purification. RESULTS The α ‐pinene‐degrading bacterium, Pseudomonas veronii ZW completely mineralized α‐pinene to CO 2 and H 2 O . One important intermediate metabolite, 4‐isopropylbenzoic acid, was chosen as the target accumulated compound. Through mutagenesis technology, the mutant, P. veronii ZW ‐A ( CCTCC M2015788 ), was obtained from the wild strain, and its metabolic pathway for α‐pinene was verified to block in the step of 4‐isopropylbenzoic acid. A 663‐bp DNA fragment flanking the transposon insertion site was obtained through self‐formed adaptor PCR , and was designated as gene orfA , displaying 96% deduced amino acid sequence – similar to rulAB ‐like protein. Since this protein contained the mono‐aromatic and polyaromatic compounds homologue degradation gene, the mutant ZW ‐A might be an orfA ‐disrupted strain, incapable of degrading 4‐isopropylbenzoic acid. Through the optimization of cultural medium, the accumulation rate increased from 19% to 30.4%. CONCLUSION This research identified the gene which was related to the degradation of 4‐isopropylbenzoic acid. Such results provided a further understanding of microbial metabolism of α‐pinene by Pseudomonas species and a new method for microbial biosynthesis of 4‐isopropylbenzoic acid. © 2017 Society of Chemical Industry