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Simultaneous production of C‐phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis
Author(s) -
Dejsungkrat Monchai,
Chisti Yusuf,
Sirisansaneeyakul Sarote
Publication year - 2017
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5293
Subject(s) - phycocyanin , pigment , biomass (ecology) , food science , light intensity , chemistry , arthrospira , light cycle , carotenoid , composition (language) , chlorophyll a , chlorophyll , botany , chromatography , biochemistry , biology , cyanobacteria , organic chemistry , linguistics , genetics , physics , philosophy , optics , neuroscience , bacteria , agronomy , circadian rhythm
BACKGROUND Arthrospira platensis produces the blue pigment C‐phycocyanin (C‐PC) and extracellular polymeric substances (EPS) of potential value in food and pharmaceutical applications. RESULTS Batch cultures were used to simultaneously produce C‐PC and EPS. The effects of incident light level on growth and product formation are reported. A maximum biomass concentration of 1.5 g L −1 in 14 days was obtained at a light intensity of 203 µmol m −2 s −1 . The light intensity affected the biomass composition and the production of the two products. Protein, phenolic compounds and chlorophyll a in the biomass were reduced at high light levels, but carbohydrate and the total carotenoids were increased. Production of C–PC was growth associated. The highest concentration of C–PC in the culture broth was ∼60 mg L −1 at a photon flux of 135 µmol m −2 s −1 . EPS was produced mostly in the stationary phase. The highest EPS concentration of ∼960 mg L −1 occurred at a photon flux of 203 µmol m −2 s −1 . Incident light level also affected the antioxidant properties of the two products. CONCLUSION Incident light strongly affected the production of C‐PC, EPS and the biomass composition. Low light favored C‐PC production whereas high light favored the production of EPS. © 2017 Society of Chemical Industry