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Biotransformation of l ‐menthol by soil‐borne plant pathogenic fungi ( Rhizoctonia solani )
Author(s) -
Miyazawa Mitsuo,
Kawazoe Hideki,
Hyakumachi Mitsuro
Publication year - 2002
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.522
Subject(s) - rhizoctonia solani , biotransformation , metabolite , rhizoctonia , chemistry , menthol , microbiology and biotechnology , stereochemistry , biology , botany , organic chemistry , biochemistry , enzyme
The biotransformation of l ‐menthol was investigated by using nine isolates of Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4 and P‐1) as a biocatalyst. In the cases of Rhizoctonia solani F‐1, F‐4 and P‐1, almost all of the substrate was consumed in 3 days and the major metabolite increased rapidly for the first of 3 days incubation. The structure of the major metabolite was elucidated on the basis of its spectral data. The major metabolite was determined to be (−)‐(1 S ,3 R ,4 S ,6 S )‐6‐hydroxymenthol which indicated that l ‐menthol was hydroxylated at the C‐6 position. From the main component analysis, the nine isolates of Rhizoctonia solani were divided into two groups based on their ability to transform l ‐menthol to (−)‐(1 S ,3 R ,4 S ,6 S )‐6‐hydroxymenthol. This is the first report on the biotransformation of l ‐menthol by Rhizoctonia solani . © 2001 Society of Chemical Industry