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Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction
Author(s) -
Gautério Gabrielle Victoria,
Malta Danielle Specht,
Reginatto Laís,
Feltrin Ana Carla Penteado,
GardaBuffon Jaqueline,
Kalil Susana Juliano
Publication year - 2017
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.5191
Subject(s) - peroxidase , chemistry , ultrafiltration (renal) , diafiltration , chromatography , enzyme , bran , biochemistry , membrane , organic chemistry , raw material , microfiltration
BACKGROUND Once peroxidase purification can improve substrate degradation capacity, peroxidase from rice bran was purified by expanded bed adsorption ( EBA ) coupled to diafiltration/ultrafiltration ( DF / UF ) and applied for deoxynivalenol ( DON ) reduction. RESULTS The DF / UF step was optimized according to membrane molecular weight cutoff ( MWCO ), DF cycles number, operation temperature and CaCl 2 addition to the DF buffer. DON reduction tests were carried out with both clarified and purified peroxidase. The use of MWCO of 10 kDa , 10 °C, six DF cycles and adding 4 mmol L −1 CaCl 2 to DF buffer promoted enzyme purification of 4.4‐fold and enzymatic recovery of 79.4%. The global purification process combining two techniques resulted in enzyme purification of 75.1‐fold with an enzymatic recovery of 22.8%. Peroxidase purified by EBA combined with DF / UF resulted in DON reduction of 81.7% in a model system. CONCLUSION Peroxidase purification was achieved by scale‐up integrated strategies. The use of purified peroxidase from agricultural by‐products could be a promising alternative for enzymatic DON levels reduction. © 2017 Society of Chemical Industry