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The enhanced production of 11α‐hydroxyandrosta‐1,4‐diene‐3,17‐dione based on the application of organic silica hollow spheres in the biotransformation of β‐sitosterol
Author(s) -
Rong Shaofeng,
Wang Jingwen,
Li Qianqian,
Guan Shimin,
Cai Baoguo,
Zhang Shuo,
Hu Jing
Publication year - 2017
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4983
Subject(s) - biotransformation , chemistry , solubility , adsorption , fourier transform infrared spectroscopy , substrate (aquarium) , organic chemistry , nuclear chemistry , chemical engineering , biology , ecology , engineering , enzyme
BACKGROUND 11α‐hydroxyandrosta‐1,4‐diene‐3,17‐dione (11α‐ OH‐ADD ) serves as an important steroid intermediate for many hormone pharmaceuticals. However, the poor aqueous solubility of the β‐sitosterol which is the substrate hinders its productivity efficiency. This paper reports a new method of biotransformation of β‐sitosterol to 11α‐ OH‐ADD by mixed cultivation of Mycobacterium MF006 and Aspergillus ochraceus MF007 with organic silica hollow spheres ( OSHS ) to improve the dispersibility of the substrate based on its excellent adsorption property. The effect of co‐cultivation method and OSHS on the biotransformation were evaluated. RESULTS Results obtained by scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis indicated that the adsorption complexes were formed with β‐sitosterol and OSHS , which increased the opportunity for interaction between microorganisms and β‐sitosterol. Using adsorption complexes as substrates, the conversion efficiency increased to 87.11% and the yield of 11α‐ OH‐ADD enhanced one and a half times through the synergy of these two microorganisms. CONCLUSION This study is the first to demonstrate that OSHS can increase the solubility of β‐sitosterol and the mixed cultivation of microorganisms is able to enhance production and shorten duration, which provides a new direction for enhancing the biotransformation efficiency of steroids. © 2016 Society of Chemical Industry