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High‐efficient xylitol production by evolved Candida maltosa adapted to corncob hemicellulosic hydrolysate
Author(s) -
Jiang Xiaolong,
He Peng,
Qi Xianni,
Lin Yuping,
Zhang Yongkui,
Wang Qinhong
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4924
Subject(s) - xylitol , corncob , xylose , bioprocess , hydrolysate , fermentation , chemistry , substrate (aquarium) , food science , yield (engineering) , biochemistry , microbiology and biotechnology , biology , hydrolysis , raw material , organic chemistry , materials science , paleontology , ecology , metallurgy
BACKGROUND To compete with chemical hydrogenation, xylitol production by a biotechnological method has to decrease production cost by improving the performance of the strain, using low‐cost substrate and developing a simple and efficient bioprocess. This study attempted to reach high‐efficient xylitol production using cheap substrate. RESULTS A new evolved strain, Candida maltosa CHH65 showed improved cell growth in corncob hemicellulosic hydrolyzate ( CHH ) and similar product performance using xylose and CHH as substrate, was developed by serial culture transfer for adapting to CHH and solving the problem of inhibition of CHH . The new strain was recycled at least four times and still produced xylitol. In the test conditions, xylitol concentration reached more than 100 g L −1 within 48 h, with yield > 0.73 g g −1 , and productivity > 2 g L −1 h −1 when using CHH as substrate. CONCLUSION The capability of the xylose‐fermenting C. maltosa to convert CHH to xylitol was greatly enhanced so that the newly developed bioprocess for xylitol production from low‐cost substrate with multiple recycled C. maltosa could be suitable for industrial application and reduce the production cost. © 2016 Society of Chemical Industry