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Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
Author(s) -
Arifin Mohd Azmir,
Mel Maizirwan,
Samsudin Nurhusna,
Hashim Yumi Zuhanis HasYun,
Salleh Hamzah Mohd.,
Sopyan Iis,
Nordin Norshariza
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4855
Subject(s) - microcarrier , polyvinyl alcohol , chemical engineering , fourier transform infrared spectroscopy , materials science , contact angle , polystyrene , ultraviolet , solvent , ultraviolet light , polymer , chemistry , organic chemistry , composite material , cell , engineering , biochemistry , optoelectronics
BACKGROUND Microcarrier based cell culture offers many advantages when compared with conventional cell cultures, and this technology has been widely used in the production of many important biological materials. In this work, microcarriers were produced using materials and methods that are relatively simple and low‐cost. RESULTS Polystyrene ( PS ) microspheres were prepared by using an emulsion solvent–evaporation method and the effects of process conditions on the size of microspheres were investigated. The mean size of microspheres decreased with increasing agitation speed, concentration of polyvinyl alcohol ( PVA ) and polymer to solvent ratios but increased with increasing oil to water phase ratios. To enhance its cell attachment abilities, surfaces of PS microspheres were loaded with oxygen functional groups by using an ultraviolet/ozone ( UVO 3 ) system. Analysis by Fourier transform infrared ( FTIR ), energy dispersive X‐ray spectroscopy ( EDX ), toluidine blue O ( TBO ) assay and relative contact angle measurements showed increased surface oxygen concentration after UVO 3 treatment. Results from spinner flask culture revealed that UVO 3 treated PS microspheres support the growth of Vero cells to high cell density. CONCLUSION UVO 3 treatment successfully rendered the hydrophobic surface of PS microspheres hydrophilic. This newly developed microcarrier could serve as a low‐cost alternative to commercial microcarriers available in the market today. © 2015 Society of Chemical Industry