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Magnetic mesoporous enzyme–silica composites with high activity and enhanced stability
Author(s) -
Cui Jiandong,
Feng Yuxiao,
Yue Shu,
Zhao Yamin,
Li Linbo,
Liu Ronglin,
Lin Tao
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4786
Subject(s) - mesoporous silica , immobilized enzyme , thermal stability , catalysis , materials science , biocatalysis , chemical engineering , magnetic nanoparticles , nanoparticle , polymer , fourier transform infrared spectroscopy , silicon dioxide , chemistry , mesoporous material , nanotechnology , organic chemistry , enzyme , composite material , engineering , ionic liquid
BACKGROUND Encapsulation of enzymes in biomimetic silica seems to be a promising immobilization method due to mild processing conditions and short preparation time. However, biomimetic silica particles may be considered too small for industrial applications because they result in difficult recovery. Furthermore, the use of R5 peptide or polymers as catalyst usually causes protein denaturation. To overcome these drawbacks, a new stable and recyclable biocatalyst based on co‐encapsulation of magnetic nanoparticles and phenylalanine ammonia lyase ( PAL ) in biomimetic silica has been prepared using enzyme‐assisted direct condensation reactions of silicon oxide with tetramethoxysilane ( TMOS ). RESULTS Under optimal conditions (10 mg mL −1 nanoparticles, 4.5 U mL −1 PAL , 0.8 mol L −1 TMOS ), the highest activity recovery (52%) of encapsulated PAL was obtained. Compared with conventional PAL encapsulated in biomimetic silica (28%), the activity recovery was increased 1.86‐fold. TEM , CLSM , magnetisation measurements, and FTIR confirmed that the magnetic nanopaticles and PAL had been co‐encapsulated into the biomimetic silica. The resulting immobilized PAL exhibited higher activity and stability than native PAL , and the immobilized PAL can be easily separated by applying a magnetic field. CONCLUSIONS This work provides a convenient strategy to prepare stable and easy recycled biocatalyst with excellent catalytic performance. © 2015 Society of Chemical Industry