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Development and characterization of a new adsorbent for biomolecule separation: intercalation and adsorption of clavulanic acid in layered double hydroxides
Author(s) -
Forte Marcus Bruno Soares,
TaviotGuého Christine,
Leroux Fabrice,
Rodrigues Maria Isabel,
Maugeri Filho Francisco
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4759
Subject(s) - adsorption , intercalation (chemistry) , coprecipitation , layered double hydroxides , hydroxide , biomolecule , porosity , chemistry , freundlich equation , particle (ecology) , particle size , chromatography , inorganic chemistry , nuclear chemistry , organic chemistry , geology , biochemistry , oceanography
BACKGROUND A new layered double hydroxide ( LDH ) material has been developed through a pioneering approach, with the goal of being an alternative adsorbent for the separation of biomolecules, such as clavulanic acid ( CA ). RESULTS Zn 2 Cr‐CA hybrid LDH assemblies were prepared through a coprecipitation method to evaluate the affinity between the CA molecule and the interlayer sites. The resulting inorganic–organic ‘sandwich’ structure was characterized by a combination of techniques showing an expansion of the layered structure from 0.78 nm up to values of 2.30 nm upon CA intercalation. Isotherm studies were carried out to evaluate the adsorption capacity of LDH towards CA . The Freundlich adsorption model was found to fit the experimental data well, indicating a relatively large adsorption capacity for the adsorbent Zn 2 Cr‐NO 3 , as high as 73.63, 128.76, and 229.62 mg 1‐ nF L nF g −1 at 20, 30, and 35 °C, respectively. CONCLUSION Zn 2 Cr‐NO 3 adsorption capacity was not influenced by the presence of amino acids, thus underlining that it is a LDH composition which may be a suitable alternative for the separation of CA . LDH micro‐particles were additionally encapsulated with calcium alginate gel ( LDH ME ) and characterized for particle average diameter ( d p = 171 µm), bulk density ( ρ bulk = 0.63 g cm −3 ), particle density ( ρ p = 2.2 g cm −3 ), particle porosity ( ϵ p = 0.71) and bed porosity ( ϵ B = 0.40). The application of LDH ME in the separation of CA from complex mixtures, containing tyrosine and proline, was considered satisfactory based on purification factors of about 2.32 and degrees of purity higher than 99%. © 2015 Society of Chemical Industry