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Evaluation of a recycling bioreactor for biosurfactant production by Pseudomonas aeruginosa MR01 using soybean oil waste
Author(s) -
Lotfabad Tayebe Bagheri,
Ebadipour Negissa,
RoostaAzad Reza
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4733
Subject(s) - bioreactor , pulp and paper industry , kerosene , distilled water , chemistry , soybean oil , environmental science , waste management , chromatography , food science , engineering , organic chemistry
BACKGROUND This study deals with the large‐scale production of biosurfactant from soybean oil soapstock by Pseudomonas aeruginosa MR01 . The production of biosurfactant was carried out in a newly designed bioreactor equipped with recycling flow under three operational conditions. Kinetic studies were conducted at both shake flask and 5‐L bioreactor scales during fermentation in a soapstock medium. Mathematical equations were developed to model the kinetic patterns at both scales. RESULT Statistical analyses demonstrated the goodness of fit, with regression r‐squared, R 2 , between 0.97and 0.99 for different models. Furthermore, biosurfactant concentration in the bioreactor including the recycling flow, which was switched on and off during a 6‐h period, reached 25.5 g L −1 ( Y P /S =0.9) which showed 41.5% and 21.5% growth compared with experiments conducted in a shake flask and a non‐recycling‐flow bioreactor, respectively. Moreover, the crude biosurfactant at a concentration of 24 mg L −1 was observed to have lowered the surface tension of double‐distilled water from 72 mN m −1 to 28.5 mN m −1 and displayed an emulsification index ( E24 ) of 63% against kerosene. CONCLUSION The results showed the recycling‐flow bioreactor to be a feasible model for further large‐scale studies of biosurfactants. © 2015 Society of Chemical Industry