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Polydopamine assisted preparation of Ti 4+ ‐decorated magnetic particles for selective and rapid adsorption of phosphorylated proteins
Author(s) -
Ma Xiangdong,
Jia Li
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4654
Subject(s) - adsorption , selectivity , phosphorylation , chemistry , protein adsorption , metal , metal ions in aqueous solution , protein purification , protein phosphorylation , chromatography , biochemistry , organic chemistry , protein kinase a , catalysis
BACKGROUND Phosphorylated proteins have attracted widespread attention due to their crucial roles in regulating biological processes. Therefore, development of effective methods for identification and detection of phosphorylated proteins is an indispensable step to understand the roles of protein phosphorylation. The aim of this work is to prepare Ti 4+ ‐decorated polydopamine‐grafted hybrid magnetic particles (Ti 4+ ‐ PDA @ Fe 3 O 4 ) for highly efficient and selective separation of phosphorylated proteins. RESULTS Ti 4+ ‐ PDA @ Fe 3 O 4 particles were synthesized and characterized. A wealth of hydroxyl groups in PDA enhanced the binding capacity of PDA @ Fe 3 O 4 for titanium ions. The selectivity of the particles for phosphorylated proteins relies on the affinity of phosphate groups in phosphorylated proteins to titanium ions on the surface of Ti 4+ ‐ PDA @ Fe 3 O 4 . The adsorption capacity of Ti 4+ ‐ PDA @ Fe 3 O 4 for β‐casein was 1273.9 mg g ‐1 . The adsorption conditions and selectivity of Ti 4+ ‐ PDA @ Fe 3 O 4 for phosphorylated proteins were studied. The feasibility of Ti 4+ ‐ PDA @ Fe 3 O 4 for separation of phosphorylated proteins from milk was also investigated. CONCLUSIONS Ti 4+ ‐ PDA @ Fe 3 O 4 demonstrated highly effective and selective adsorption property for phosphorylated proteins based on immobilized metal affinity chromatography mechanism. The successful isolation of phosphorylated proteins from milk by Ti 4+ ‐ PDA @ Fe 3 O 4 demonstrated that the particles will have promising application prospect in selective separation of phosphorylated proteins. © 2015 Society of Chemical Industry