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Synthesis of polyethyleneimine ( PEI ) and β ‐cyclodextrin grafted PEI nanocomposites with magnetic cores for lipase immobilization and esterification
Author(s) -
Khoobi Mehdi,
KhalilvandSedagheh Masoud,
Ramazani Ali,
Asadgol Zahra,
Forootanfar Hamid,
Faramarzi Mohammad Ali
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4579
Subject(s) - polyethylenimine , nanocomposite , thermogravimetric analysis , lipase , thermal stability , magnetic nanoparticles , fourier transform infrared spectroscopy , immobilized enzyme , cyclodextrin , nuclear chemistry , materials science , chemical engineering , surface modification , chemistry , nanoparticle , organic chemistry , nanotechnology , transfection , biochemistry , engineering , gene , enzyme
BACKGROUND Fabrication of nanocomposites with a magnetic core and their applications for enzyme immobilization has been introduced as an intresting trend during recent decades. The present study described the synthesis of polyethyleneimine ( PEI ) and β ‐cyclodextrin grafted PEI nanocomposites with magnetic cores and their applications for lipase immobilization. Fe 3 O 4 magnetic nanoparticles ( MNPs ) were prepared by co‐precipitation of Fe ( III ) and Fe ( II ) ions and then coated with polyethylenimine and/or hydroxyapatite/polyethylenimine, followed by grafting β ‐cyclodextrin onto the surface of the prepared nanocomposites. The nanocomposites were then characterized using Fourier transform infrared spectroscopy, X‐ray diffraction, thermogravimetric analysis, vibrating sample magnetometry, and scanning and transmission electron microscopy and consequently applied for the immobilization of lipase. RESULTS The results obtained for thermal and pH stability, reusability, and storage stability showed that the MNPs @hydroxyapatite/polyethylenimine/ β ‐cyclodextrin was the best nanocomposite for lipase immobilization. Application of the immobilized lipase onto the selected nanocomposite for the synthesis of ethyl valerate (green apple flavor) revealed esterification rates of 79% and 62% in n ‐hexane and dimethyl sulfoxide, respectively, after 24 h incubation. CONCLUSION The results obtained demonstrate good stability, reusability, and potential application of immobilized lipase on magnetic nanocomposite of MNPs @hydroxyapatite/polyethylenimine/ β ‐cyclodextrin for synthesis of ethyl valerate especially in organic medium. © 2014 Society of Chemical Industry