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Biodesulfurization of dibenzothiophene by resting cells of Pseudomonas putida CECT5279 : influence of the oxygen transfer rate in the scale‐up from shaken flask to stirred tank reactor
Author(s) -
Martinez Igor,
Santos Victoria E,
Gomez Emilio,
GarciaOchoa Felix
Publication year - 2016
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4559
Subject(s) - bioreactor , dibenzothiophene , continuous stirred tank reactor , pseudomonas putida , chemistry , oxygen , mass transfer , volumetric flow rate , scale up , limiting oxygen concentration , chemical engineering , mass transfer coefficient , work (physics) , oxygen transport , thermodynamics , chromatography , organic chemistry , flue gas desulfurization , engineering , physics , classical mechanics , enzyme
BACKGROUND In this work, the scale‐up of the biodesulfurization process at rest from shaken flask to stirred tank bioreactor cells has been studied taking into account the influence of the hydrodynamic conditions of the oxygen transfer rate. RESULTS Different hydrodynamic conditions to carry out the biodesulfurization in a stirred tank bioreactor have been studied and compared with the results obtained in a shaken flask. The best results were obtained at a stirrer speed of 400 rpm and an air flow rate of 2 vvm. The oxygen transfer rate has been found to be the rate‐limiting step, and there is a critical value, determined by a value for the volumetric mass transfer coefficient ( k L a ) of around 1.4 × 10 −2 s −1 , which cannot be exceeded without affecting the biodesulfurization process. CONCLUSION The 4S biodesulfurization pathway is highly sensitive to oxygen availability, even under oxygen limiting conditions. Results obtained in a shaken flask have been reproduced satisfactorily in a stirred tank bioreactor by combining different hydrodynamic conditions and the constant‐ k L a scale‐up criterion has been used to validate this approach. © 2014 Society of Chemical Industry