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Biphasic recognition enantioseparation of ofloxacin enantiomers by an aqueous two‐phase system
Author(s) -
Jiao Feipeng,
Wang Jun,
Jiang Xinyu,
Yang Hua,
Shi Shuyun,
Chen Xiaoqing,
Yu Jingang
Publication year - 2015
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4538
Subject(s) - enantiomer , chemistry , extraction (chemistry) , ofloxacin , chromatography , aqueous solution , ammonium sulfate , tartaric acid , cyclodextrin , enantioselective synthesis , aqueous two phase system , mass fraction , high performance liquid chromatography , organic chemistry , biochemistry , catalysis , ciprofloxacin , citric acid , antibiotics
BACKGROUND Our previous study indicated that monophasic recognition enantioseparation based on an aqueous two‐phase system ( ATPS ) is restricted because of its relatively low enantioselectivity. To improve the enantioselectivity, biphasic chiral selectors could be introduced into the ATPS . This work has focused on ATPS technology utilizing cooperation of L ‐tartaric acid ( L ‐ TA ) with hydroxypropyl‐ β ‐cyclodextrin ( HP ‐ β ‐ CD ), for the biphasic recognition enantioseparation of ofloxacin ( OFLX ) enantiomers. RESULTS Under the optimum conditions, namely PEG2000 mass fraction of 40% (w/w), ammonium sulfate mass fraction 25% (w/w), pH value 4, HP ‐β‐ CD concentration 0.02 mol L −1 , L ‐ TA concentration 0.05 mol L −1 , separation factor ( α ) reached 1.32 after just one‐step extraction. Furthermore, for the purpose of explaining the extraction behavior, a reasonable enantioselective extraction mechanism was also proposed. CONCLUSION The novel biphasic recognition extraction based on ATPS presented in this work has the ability for good enantioseparation of OFLX enantiomers. © 2014 Society of Chemical Industry