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Selective reverse micellar extraction of penicillin acylase from E coli
Author(s) -
Gaikar Vilas G,
Kulkarni Madhav S
Publication year - 2001
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.444
Subject(s) - periplasmic space , sonication , chemistry , penicillin amidase , extraction (chemistry) , penicillin , chromatography , micellar solutions , escherichia coli , cell disruption , micelle , biochemistry , antibiotics , organic chemistry , aqueous solution , gene
The recovery of penicillin acylase from E coli by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the periplasmic space of E coli without disrupting the cells. Unlike ultrasonication which breaks open the cells entirely, making subsequent processing difficult and expensive, reverse micellar treatment of cells gave a moderate recovery of 60% of enzyme activity in a highly pure form. © 2001 Society of Chemical Industry