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Development of parallel miniature bubble column bioreactors for fermentation process
Author(s) -
Kheradmandnia Soheila,
HashemiNajafabadi Sameereh,
Shojaosadati Seyed Abbas,
Mousavi Seyyed Mohammad,
Malek Khosravi Khosro
Publication year - 2015
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4408
Subject(s) - bioreactor , aeration , scale up , bioprocess , bubble column reactor , bubble , process engineering , growth rate , chromatography , fermentation , mass transfer , evaporation , reproducibility , mixing (physics) , process development , chemistry , materials science , pulp and paper industry , chemical engineering , mathematics , thermodynamics , mechanics , engineering , physics , biochemistry , organic chemistry , classical mechanics , quantum mechanics , gas bubble , geometry
BACKGROUND In recent years, bioprocess development has been used scaled down bioreactors, parallel operation and on‐line monitoring. In this study novel miniature bubble column bioreactors (MBCRs) have been developed. Reproducibility of the bioreactors, the effect of operational parameters and scale up to a conventional STR were studied.RESULTS Fermentations in the parallel MBCRs were reproducible under the same conditions. Increasing the aeration rate had an insufficient effect on the specific growth rate ( μ ) of E. coli , but it led to an increase in the volumetric mass transfer coefficient ( K L a ) and evaporation rate. The oxygen uptake rate (OUR) remained constant at different aeration rates as well as μ . Increasing the temperature resulted in a significant increase in μ , K L a , evaporation rate and OUR. Control of the pH had a slight but sufficient effect on K L a . Scale up results with an equal K L a demonstrated good agreement between the MBCR and a conventional STR. According to the Damköhler number, the biochemical reaction was rate‐limited.CONCLUSION High achievable K L a in the MBCRs and successful scale‐up suggested the present system was an efficient alternative to the stirred or shaken devices for high throughput cultivations. © 2014 Society of Chemical Industry