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Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan–metal nanocomposites
Author(s) -
Kaur Pawan,
Thakur Rajesh,
Barnela Manju,
Chopra Meenu,
Manuja Anju,
Chaudhury Ashok
Publication year - 2015
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4383
Subject(s) - nuclear chemistry , chitosan , chemistry , nanocomposite , antimicrobial , zinc , aspergillus flavus , fourier transform infrared spectroscopy , biopolymer , materials science , organic chemistry , chemical engineering , food science , nanotechnology , engineering , polymer
BACKGROUND The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material. RESULTS Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate ( CuSO 4 . 5H 2 O ) and zinc acetate (Zn ( O 2 CCH 3 ) 2 ) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy ( TEM ), Fourier transform infrared ( FTIR ) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses ( SEM‐EDX ) and differential scanning calorimetry ( DSC ). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro . Antifungal activity was also studied against Alternaria alterneta , Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method. CONCLUSIONS It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry