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Long‐term production of butyric acid through immobilization of Clostridium tyrobutyricum in a moving fibrous‐bed bioreactor ( MFBB )
Author(s) -
Shi Zhouming,
Huang Lei,
Wu Xiangting,
Luo Lanping,
Xiao Kaijun,
Cai Jin,
Xu Zhinan
Publication year - 2014
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4271
Subject(s) - butyric acid , bioreactor , fermentation , bioprocess , chemistry , lactic acid , chromatography , yield (engineering) , chemical engineering , food science , materials science , bacteria , organic chemistry , biology , composite material , engineering , genetics
BACKGROUND Butyric acid is an important chemical with wide industrial applications, but large‐scale bio‐production still requires an efficient bioprocess. For this purpose, one novel moving fibrous‐bed bioreactor ( MFBB ) was developed in this work for economical production of butyric acid. RESULTS The cells of Clostridium tyrobutyricum were immobilized on a fibrous matrix packed in porous levitated sphere carriers. Each carrier was packed with a fiber quantity 2 cm × 6 cm (0.42 g) and was rotated at 100 rpm in a stirred‐tank fermentor having pH and temperature controls. Batch and repeated‐batch fermentations were carried out to produce butyric acid with a high yield (0.48 ± 0.015 g g ‐1 ). Subsequent fed‐batch fermentation using the MFBB resulted in a high concentration of butyric acid (65.4 ± 1.4 g L ‐1 ). This novel MFBB could be employed to produce high concentration butyric acid (64.5 ± 1.2 g L ‐1 ) by a simple repeated‐batch addition of a high concentration of glucose (>100 g L ‐1 ). CONCLUSIONS The MFBB system developed could practically and economically produce butyric acid from glucose for long‐term operation. © 2013 Society of Chemical Industry